Research Use Only. This guide is intended for in vitro laboratory research purposes only. All Zen Well Labs peptides are sold exclusively for research use and are not intended for human or veterinary consumption.
Required Supplies
Lyophilized Peptide Vial *
Your research peptide in freeze-dried powder form
Bacteriostatic Water (BAC Water) *
0.9% benzyl alcohol in sterile water — the preferred reconstitution medium
Sterile Laboratory Transfer Syringes *
For precise volume transfer between vials — standard laboratory supply
Alcohol Swabs (70% IPA) *
For sanitising vial stoppers before transfer
Clean Work Surface *
Sterile or alcohol-wiped surface to work on
Refrigerator (2-8°C) *
For storing reconstituted peptide solution
Label & Marker
To record reconstitution date and concentration on vial
Calculator
For concentration calculations
Concentration Reference Table
Use this table to determine how much BAC Water to add to achieve your desired concentration. Formula: Volume (mL) = Peptide (mg) ÷ Concentration (mg/mL)
| Peptide Amount | BAC Water Added | Final Concentration | Per 0.1mL Draw |
|---|---|---|---|
| 5 mg | 5 mL | 1 mg/mL (1000 mcg/mL) | 100 mcg |
| 5 mg | 2.5 mL | 2 mg/mL (2000 mcg/mL) | 200 mcg |
| 5 mg | 1 mL | 5 mg/mL (5000 mcg/mL) | 500 mcg |
| 2 mg | 2 mL | 1 mg/mL (1000 mcg/mL) | 100 mcg |
| 2 mg | 1 mL | 2 mg/mL (2000 mcg/mL) | 200 mcg |
| 10 mg | 10 mL | 1 mg/mL (1000 mcg/mL) | 100 mcg |
Step-by-Step Reconstitution Protocol
Prepare Your Work Area
Wipe your work surface with a 70% isopropyl alcohol swab and allow to dry. Wash hands thoroughly. Gather all supplies before beginning.
Calculate Your Volume
Determine the volume of BAC Water needed based on your desired concentration. Refer to the table above. Write this down before proceeding.
Sanitise Both Vial Stoppers
Wipe the rubber stopper of both the peptide vial and the BAC Water vial with separate alcohol swabs. Allow to air dry for 30 seconds — do not blow on them.
Draw the BAC Water
Using a sterile laboratory transfer syringe, carefully draw the calculated volume of bacteriostatic water from the BAC Water vial. Remove any air bubbles by gently tapping the syringe and expelling air.
Transfer Slowly Down the Vial Wall
Insert the transfer syringe through the peptide vial septum at an angle. Slowly dispense the BAC Water down the inner glass wall of the vial — never directly onto the lyophilized powder cake. This prevents denaturation from mechanical force.
Dissolve Gently — Do Not Shake
Gently swirl the vial in slow, circular motions until the powder is completely dissolved. This may take 30-60 seconds. Do not shake, vortex, or agitate vigorously — this can cause aggregation and denaturation.
Inspect the Solution
The reconstituted solution should be clear and colourless (or very slightly yellow for some peptides). Discard immediately if the solution is cloudy, contains particulates, or has an unusual colour.
Label and Store
Label the vial with the peptide name, concentration, reconstitution date, and your initials. Store at 2-8°C (refrigerator). Do not freeze reconstituted peptide. Use within 28-30 days.
Storage Guidelines
Lyophilized (Unreconstituted)
Store away from light and moisture. Do not refrigerate long-term.
Reconstituted Solution
Do not freeze. Keep away from light. Label with date.